The ligand-binding domain (LBD/148-410) of the thyroid hormone receptor alpha 1 (TRα1) was expressed in the E coli system and purified by the combination of Ni-affinity column and other conventional chromatographic methods. The resulted protein contains 275 amino acids with a 6His tag at the N-terminus. It migrates in SDS-PAGE with an apparent molecular mass of 32kDa.
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