Recombinant human SP1 protein (GC box-binding protein) was expressed in the baculovirus system and purified by an affinity column in combination of other chromatograph methods. The resulted protein is a monomeric polypeptide of 792 (14+778) amino acids with a 6His tag at the N-terminus. It migrates in SDS-PAGE with an apparent molecular mass of 95kDa
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