Human Positive Cofcator 4 (PC4) was purified from HeLa cells and capable of enhancing
activated transcription by diverse gene- or/and tissue-specific DNA-binding nuclear protein.
Phosphorylation of PC4 at its N-terminal domain negatively regulates its ability to bind to
activators and DNA. Recent studies have demonstrated that PC4 is one of the common cancer
markers. Its expression correlates well with tumorigenesis of most common tumors.
Recombinant human PC4 was expressed in E coli and purified by the combination of
conventional and FPLC chromatography methods to >95% homogeneity. It has been tested
for its activity in different assays including in vitro transcription, gel mobility shift assay and
DNA-binding assay.

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