Recombinant Human DNA Polymerase α

Recombinant human DNA polymerase alpha was expressed in Sf9 cells and purified by an
affinity column in combination of other chromatograph methods. The resulted protein is a
monomeric polypeptide of 1474 (1462+12) amino acids with a 6His tag at the C-terminus. It
migrates in SDS-PAGE with an apparent molecular mass of 170kDa.

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