Recombinant human poly (ADP-ribose) polymerase I (PARP1) was expressed in Sf9 cells and purified by an affinity column in combination of other chromatograph methods. The resulted protein is a monomeric polypeptide of 1028 (1014+14) amino acids with a 6His tag at the Cterminus. It migrates in SDS-PAGE with an apparent molecular mass of 120kDa.
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